Anti-Human Ig Lambda Light Chain Rabbit Monoclonal Antibody [Clone RM127]; 31-1029-00
![Anti-Human Ig Lambda Light Chain Rabbit Monoclonal Antibody [Clone RM127]; 31-1029-00](/upload/image/products/RM127_image3-e1453506680837.jpg)
![Anti-Human Ig Lambda Light Chain Rabbit Monoclonal Antibody [Clone RM127]; 31-1029-00](/upload/image/products/Fig1_HuLambda_e.png)
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Product Details
| Product Name | Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody, Clone RM127 |
| Description | Rabbit monoclonal to Human Lambda Light Chain |
| Catalog No. | 31-1029-00 |
| Clone Name | RM127 |
| Product Data Sheet | Anti-Human Lambda Light Chain Rabbit Monoclonal Antibody Data Sheet RM127 |
| Specificity | RM127 reacts to the lambda light chain of human immunoglobulins, and does not react to the kappa light chain. RM127 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG |
| Application | Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.05 µg/mL – 0.2 µg/mL; Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL; Flow Cytometry (FC): 0.2 µg/mL - 2 µg/mL. |
| Keywords | |
| References for Human Ig Lambda Light Chain antibody [RM127] | Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550) |
 | Immunohistochemical staining of formalin fixed andparaffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonalantibody, Clone RM127. |
 | ELISA showing RM127 reacts only to the lambda light chain of human immunoglobulins, and not to the kappa light chain, mouse IgG, rat IgG, or goat IgG. |
 | A titer ELISA using RM127. The plate was coated with different amounts of human IgG3λ. A serialdilution of RM127 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody. |
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