5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair [RM236/RM476], 31-1111-MK

Key features and details

  • Uniprot: N/A
  • Gene ID: N/A
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1) Capture Antibody: Anti-5-Hydroxymethylcytosine Rabbit Monoclonal Antibody, Clone RM236, 50 ug at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins. 2) Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
  • Brand:
CAT.NO. : 31-1111-MK
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Product Details
Product Details
Product Name5-Hydroxymethylcytosine (5-hmC) DNA Matched Antibody Pair [RM236/RM476]
Product DescriptionThis antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA. This product includes capture and detection antibodies for Sandwich ELISA assays.
Catalog No.31-1111-MK
Clone NameRM236 Anti-5-hydroxymethylcytosine (5-hmC) Rabbit Monoclonal Antibody; BSA Free

RM476 Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody
Product Data Sheet5-Hydroxymethylcytosine DNA Matched Antibody Pair Datasheet
SpecificityThis antibody pair detects 5-hydroxymethylcytosine in denatured DNA (single strand DNA). It does not react to non-methylated cytosine and other modified cytosine (5-mC, 5-caC, or 5-fC) in DNA.
ApplicationSandwich ELISA, or other Sandwich Assays
Species ReactivityHuman
ConjugateRM476; Biotin
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Components1)Capture Antibody: Anti-5-Hydroxymethylcytosine Rabbit Monoclonal Antibody, Clone RM236, 50 μg at 1.0 mg/mL in 50% Glycerol/PBS with 0.09% sodium azide, without BSA or other carrier proteins.
2)Detection Antibody: Biotin Anti-Adenine and Guanine Rabbit Monoclonal Antibody, Clone RM476, 25 μg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;

PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
UsageELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.2 μg/mL – 1 μg/mL
Keywords
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Product Images

Detection of 5-Hydroxymethylcytosine in mouse different tissues genomic DNA.
Sandwich ELISA using anti-5-Hydroxymethylcytosine (Clone RM236) as the capture
antibody (50ng/well at 1 μg/mL), and biotinylated anti-Adenine and Guanine
(Clone RM476)as the detection antibody (0.2 μg/mL), followed by an AP
conjugated streptavidin.

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