Anti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody [Clone RM477], 31-1369-00

Key features and details

  • Uniprot: N/A
  • Gene ID: N/A
  • Host Species: Rabbit
  • Species Reactivity: All Vertebrates
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1369-00
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Product Details
Product Details
Product NameAnti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, Clone RM477
Product DescriptionRabbit monoclonal to 5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody, 5'-fC
Catalog No.31-1369-00
Clone NameRM477
Product Data SheetAnti-5-Formylcytosines (5-fC) Rabbit Monoclonal Antibody Data Sheet RM477
SpecificityRM477 reacts to 5-Formylcytosines (5-fC) in DNA. No cross reactivity with non-modified cytosine and other modified cytosines in DNA.
ImmunogenKLH-conjugated 5-Formylcytosines.
ApplicationDot Blot
ELISA
Species ReactivityAll
ConjugateNone
Intended UseResearch Use Only
Related Product5-Formylcytosines (5-fC) DNA Matched Antibody Pair
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt.
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size50 μg
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageDot Blot: 0.5 μg/mL - 2 μg/mL
ELISA: 0.5 μg/mL - 2 μg/mL
Keywords
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Product Images

Dot blot of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477.
The membrane was pre-spotted with 50, 5, and 0.5 ng/dot of linear denatured
DNA (426 bp) containing either normal cytosines (unmodified), 5-methylcytosines
(5-mC), 5-hydroxymethylcytosines(5-hmC), 5-carboxylcytosines (5-caC), or
5-formylcytosines (5-fC).

ELISA of DNA using anti-5-fC Rabbit Monoclonal Antibody, Clone RM477 (1 µg/mL).
The plate was coated with different concentrations of linear denatured DNA (426 bp)
containing either normal cytosines (unmodified), 5-methylcytosines (5-mC),
5-hydroxymethylcytosines (5-hmC), 5-carboxylcytosines (5-caC), or
5-formylcytosines (5-fC).

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