anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
-
Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes ACACB / Acetyl Coenzyme A Carboxylase 2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Acetyl-CoA Carboxylase Beta |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human ACACB / Acetyl Coenzyme A Carboxylase 2. (EENPEVAVDCVIYLSQHISPAERAQVVHLLSTMD) |
| 偶联标记 | Un-conjugated |
| 別名 | Acetyl-CoA carboxylase 2; ACC-beta; HACC275; EC 6.4.1.2; EC 6.3.4.14; ACCB; ACC2 |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 280 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | ACACB |
| 全名 | acetyl-CoA carboxylase beta |
| 背景介绍 | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008] |
| 生物功能 | Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed:16854592, PubMed:19236960, PubMed:20457939, PubMed:20952656, PubMed:19900410, PubMed:26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:19236960, PubMed:20457939, PubMed:20952656, PubMed:26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity). [UniProt] |
| 细胞定位 | Mitochondrion. Nucleus. Endomembrane system. Note=May associate with membranes. [UniProt] |
| 预测分子量 | 277 kDa |
| 翻译后修饰 | Phosphorylated by AMPK, leading to inactivation of the enzyme. Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity). [UniProt] |
ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue. Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0). The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody WB image
Western blot: 50 µg of samples under reducing condition. Rat skeletal muscle and Mouse skeletal muscle lysates stained with ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody at 0.5 µg/ml dilution, overnight at 4°C.
ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody FACS image
Flow Cytometry: HL-60 cells were blocked with 10% normal goat serum and then stained with ARG42698 anti-ACACB / Acetyl Coenzyme A Carboxylase 2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
New Products
