Anti-Acetyl-Histone H2.AZ (Lys4) Rabbit Monoclonal Antibody, Histone H2A.Z (Acetyl Lys4) [Clone RM221], 31-1101-00
![Anti-Acetyl-Histone H2.AZ (Lys4) Rabbit Monoclonal Antibody, Histone H2A.Z (Acetyl Lys4) [Clone RM221], 31-1101-00](/upload/image/products/Fig-3_ICC_H2AZK4ac.png)
![Anti-Acetyl-Histone H2.AZ (Lys4) Rabbit Monoclonal Antibody, Histone H2A.Z (Acetyl Lys4) [Clone RM221], 31-1101-00](/upload/image/products/RM221_Fig1_E1.png)
![Anti-Acetyl-Histone H2.AZ (Lys4) Rabbit Monoclonal Antibody, Histone H2A.Z (Acetyl Lys4) [Clone RM221], 31-1101-00](/upload/image/products/RM221_Fig2-WB_E.png)
Key features and details
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CAT.NO. : 31-1101-00
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Product Details
Product Details
| Product Name | Anti-Acetyl Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody, Clone RM221 |
| Product Description | Rabbit monoclonal to Acetyl Histone H2A.Z (Lys4) |
| Catalog No. | 31-1101-00 |
| Clone Name | RM221 |
| Product Data Sheet | Anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody Data Sheet RM221 |
| Specificity | RM221 reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys4). |
| Application | Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL – 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.5 µg/mL; Immunocytochemistry: 1 µg/mL - 2 µg/mL. |
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Product Images
 | RM221 specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A. |
 | Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using RM221 at 0.5 ug/mL, showed a band of histone H2A.Z acetylated at Lysine 4 in treated HeLa. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H2A.Z (Lys4) Rabbit Monoclonal Antibody, Clone RM221 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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