Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S
![Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S](/upload/image/products/Fig2_RM156_WB.png)
![Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S](/upload/image/products/Fig1-RM156.png)
![Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S](/upload/image/products/Fig4e.png)
![Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S](/upload/image/products/Fig3e.png)
![Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [Clone RM156]; H3K79ac; Histone H3 (K79 acetylation); 31-1052-00; 31-1052-00-S](/upload/image/products/Fig5_RM156_ICC.png)
Key features and details
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Product Details
| Product Name | Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM156 |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 79 (K79ac), H3K79ac, Histone H3 (K79 acetylation) |
| Catalog No. | 31-1052-00 |
| Clone Name | RM156 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys79 Rabbit Monoclonal Antibody Data Sheet RM156 |
| Specificity | RM156 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys79). |
| Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC: 0.5 µg/mL - 2 µg/mL; ChIP: 2 µg/mL- 10 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.2 µg/mL. |
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| References | Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854) |
 | RM156 specifically reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), or Lysine 122 (K122) in Histone H3. |
 | Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM156 at 1.0 µg/mL, showed a band of histone H3 acetylated at Lysine 79 in treated HeLa. |
 | Sandwich ELISA against acetylated histone H3 at Lys 79 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3K79ac (RM156, 5 µg/mL) as the capture antibody and biotinylated anti-H3CT (RM188, 1 µg/mL) as the detection antibody. |
 | ChIP performed on HeLa cells with or without Sodium Butyrate treatment, using H3K79Ac antibody (RM156, 5 µg). Real-time PCR was performed using primers specific to the gene indicated. |
 | Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys79) Rabbit mAb RM156 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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