anti-ACOT8 antibody [4D10]

CAT.NO. : ARG57045

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Size:

50 μl

Product Details

概述

产品描述	Mouse Monoclonal antibody [4D10] recognizes ACOT8
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	4D10
同位型	IgG2a, kappa
靶点名称	ACOT8
抗原物种	Human
抗原	Recombinant fragment around aa. 1-319 of Human ACOT8.
偶联标记	Un-conjugated
別名	hACTE-III; hTE; Choloyl-coenzyme A thioesterase; EC 3.1.2.27; Peroxisomal acyl-coenzyme A thioester hydrolase 1; HNAACTE; PTE1; PTE2; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE-2; PTE-1; Thioesterase II; hACTEIII; HIV-Nef-associated acyl-CoA thioesterase; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	Assay-dependent

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 10005 Human ACOT8 Swiss-port # O14734 Human Acyl-coenzyme A thioesterase 8
基因名称	ACOT8
全名	acyl-CoA thioesterase 8
背景介绍	The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
生物功能	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation. [UniProt]
预测分子量	36 kDa

检测图片 (2)

ARG57045 anti-ACOT8 antibody [4D10] FACS image
Flow Cytometry: CTLL-2 cell line stained with ARG57045 anti-ACOT8 antibody [4D10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG57045 anti-ACOT8 antibody [4D10] WB image
Western blot: 1) 10 ng of Recombinant Human ACOT8, 40 µg of 2) Ramos cell lysate, 3) A431 cell lysate, 4) 293T cell lysate, 5) A549 cell lysate stained with ARG57045 anti-ACOT8 antibody [4D10] at 1:1000.

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