anti-AHSG antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes AHSG
  • 反应物种: Hu, Ms
  • 应用: FACS, ICC/IF, IHC-P, IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: AHSG
  • 抗原物种: Human
  • 抗原: KLH-conjugated synthetic peptide corresponding to aa. 247-276 (C-terminus) of Human AHSG.
  • Brand:
CAT.NO. : ARG55425
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes AHSG
反应物种Hu, Ms
应用FACS, ICC/IF, IHC-P, IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称AHSG
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 247-276 (C-terminus) of Human AHSG.
偶联标记Un-conjugated
別名A2HS; Alpha-2-HS-glycoprotein; HSGA; Ba-alpha-2-glycoprotein; AHS; FETUA; Alpha-2-Z-globulin; Fetuin-A
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-P1:50 - 1:100
IPAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Human liver
属性
形式Liquid
纯化Purification with Protein A and immunogen peptide.
缓冲液PBS and 0.09% (W/V) Sodium azide
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11625 Mouse AHSG

GeneID: 197 Human AHSG

Swiss-port # P02765 Human Alpha-2-HS-glycoprotein

Swiss-port # P29699 Mouse Alpha-2-HS-glycoprotein

基因名称AHSG
全名alpha-2-HS-glycoprotein
背景介绍Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008]
生物功能Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. [UniProt]
细胞定位Secreted.
研究领域Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
预测分子量39 kDa
翻译后修饰Phosphorylated by FAM20C in the extracellular medium.
O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor).
检测图片 (4)
  • ARG55425 anti-AHSG antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG55425 anti-AHSG antibody (green). DAPI (blue) for nuclear staining.

  • ARG55425 anti-AHSG antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55425 anti-AHSG antibody.

  • ARG55425 anti-AHSG antibody WB image

    Western blot: 35 µg of Human liver lysate stained with ARG55425 anti-AHSG antibody at 1:1000 dilution.

  • ARG55425 anti-AHSG antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG55425 anti-AHSG antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.

参考文献

N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets

IHC-P, IP, WB / Mouse

Guoqing Zhu et al.
Front Oncol.,  (2021)

publication_link

 

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