anti-AlaRS / Alanyl tRNA Synthetase antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG43631
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes AlaRS / Alanyl tRNA Synthetase |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | AlaRS / Alanyl tRNA Synthetase |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment corresponding to a.a. R729-N968 of Human Alanyl-tRNA Synthetase. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Alanine--tRNA ligase, cytoplasmic |
| 別名 | Renal carcinoma antigen NY-REN-42; CMT2N; EIEE29; AlaRS; EC 6.1.1.7; Alanine--tRNA ligase, cytoplasmic; Alanyl-tRNA synthetase |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa; K562; HepG2 cells | ||||||||
| 实际分子量 | 110-120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | AARS |
| 全名 | alanyl-tRNA synthetase |
| 背景介绍 | The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. [UniProt] |
| 细胞定位 | Cytoplasm [UniProt] |
| 预测分子量 | 107 kDa |
| 翻译后修饰 | ISGylated. [UniProt] |
检测图片 (3)
ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody ICC/IF image
Immunofluorescence: MCF-7 cells were stained with ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody at 1:100 dilution, 30 minutes at 37°C.
ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody WB image
Western blot: HeLa cell lysates stained with ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody, at 1:1000 dilution.
ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody FACSimage
Flow Cytometry: K562 cells were stained ARG43631 anti-AlaRS / Alanyl tRNA Synthetase antibody in 1x PBS/1% BSA for 30 min at RT.
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