anti-alpha Actinin 3 antibody [9B5]
![anti-alpha Actinin 3 antibody [9B5]](/upload/image/products/ARG40285_WB_1_210_205.jpg)
![anti-alpha Actinin 3 antibody [9B5]](/upload/image/products/ARG40285_IHC-P_1.jpg)
![anti-alpha Actinin 3 antibody [9B5]](/upload/image/products/ARG40285_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG40285
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [9B5] recognizes alpha Actinin 3 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 9B5 |
| 同位型 | IgG1 |
| 靶点名称 | alpha Actinin 3 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 574-617 of Human alpha Actinin 3. (EADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINTK) |
| 偶联标记 | Un-conjugated |
| 別名 | Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein; Alpha-actinin-3 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 100 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACTN3 |
| 全名 | actinin, alpha 3 (gene/pseudogene) |
| 背景介绍 | This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014] |
| 生物功能 | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. [UniProt] |
| 预测分子量 | 103 kDa |
检测图片 (2)
ARG40285 anti-alpha Actinin 3 antibody [9B5] IHC-P image
Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40285 anti-alpha Actinin 3 antibody [9B5] at 1 µg/ml, overnight at 4°C.
ARG40285 anti-alpha Actinin 3 antibody [9B5] WB image
Western blot: 50 µg of samples under reducing conditions. Rat skeletal muscle and Mouse skeletal muscle lysates stained with ARG40285 anti-alpha Actinin 3 antibody [9B5] at 0.5 µg/ml, overnight at 4°C.
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