anti-alpha smooth muscle Actin antibody [SQab18108]

Key features and details

  • 产品描述: Recombinant Rabbit Monoclonal antibody [SQab18108] recognizes alpha smooth muscle Actin
  • 反应物种: Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh
  • 应用: FACS, ICC/IF, IHC-Fr, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Monoclonal
  • 克隆号: SQab18108
  • 同位型: IgG
  • 靶点名称: alpha smooth muscle Actin
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66381
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Product Details
概述
产品描述Recombinant Rabbit Monoclonal antibody [SQab18108] recognizes alpha smooth muscle Actin
反应物种Hu, Ms, Rat, AGMK, Bov, Ctl, Dog, Pig, Zfsh
应用FACS, ICC/IF, IHC-Fr, IHC-P, WB
宿主Rabbit
克隆Monoclonal
克隆号SQab18108
同位型IgG
靶点名称alpha smooth muscle Actin
抗原物种Human
抗原Synthetic peptide corresponding to residues on the N-terminus of Human alpha smooth muscle Actin.
偶联标记Un-conjugated
別名Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6
应用说明
应用建议
应用推荐稀释比
FACS1:20 - 1:100
ICC/IF1:80 - 1:200
IHC-FrAssay-dependent
IHC-P1:2000 - 1:4000
WB1:1000 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂0.01% Sodium azide
稳定剂40% Glycerol and 0.05% BSA
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11475 Mouse ACTA2

GeneID: 515610 Bovine ACTA2

GeneID: 59 Human ACTA2

基因名称ACTA2
全名actin, alpha 2, smooth muscle, aorta
背景介绍The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
生物功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
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研究领域Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
预测分子量42 kDa
翻译后修饰Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).

Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.

(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). [UniProt]
检测图片 (8)
  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-Fr image

    Immunohistochemistry: Mouse liver and Mouse aorta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:100 dilution.

    From Masahiro Terasawa et al. Cells (2023), doi: 10.3390/cells12222666, Fig. 2A.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 30 μg of NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cell lysates were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution, overnight at 4°C.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] ICC/IF image

    Immunofluorescence: NRK-49F cells treated with TGF beta 1 (10 ng/ml) for 0~48 hours. Cells were fixed with 4% PFA for 15 min at room temperature and permeabilizated by 0.5% Triton X-100. Cells were stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:200 dilution, overnight at 4°C.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] (red) at 1:100 dilution in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (green) was used as a control.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded placenta stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of HeLa, HaCat, 293, LnCap, PC-3 and T47D cell lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of MDCK, MDBK, Pig heart, Zebrafish and Cos-7 lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

  • ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] WB image

    Western blot: 10 µg of Mouse brain, Rat kidney and Rat liver lysates stained with ARG66381 anti-alpha smooth muscle Actin antibody [SQab18108] at 1:2000 dilution.

参考文献

Formononetin ameliorates isoproterenol induced cardiac fibrosis through improving mitochondrial dysfunction

WB / Mouse

Lei Qian et al.
Biomed Pharmacother.,  (2024)

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Melatonin alleviates aging-related heart failure through melatonin receptor 1A/B knockout in mice

IHC-P / Mouse

Zhenyu Feng et al.
Heliyon,  (2024)

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PCSK9 inhibitors ameliorate arterial stiffness in ACS patients: evidences from mendelian randomization, cohort studies and basic experiments

WB / Mouse

Linghao Xu et al.
Preprint,  (2024)

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Mac-1 deficiency ameliorates pressure overloaded heart failure through inhibiting macrophage polarization and activation

IHC-P / Mouse

Qiu-Yue Lin et al.
BBA - Molecular Basis of Disease,  (2024)

publication_link

 

hr_line

Expression of elastin, FBXW2, fibrillin-1, and α-smooth muscle actin in damaged blood vessels of bovine legs

IHC-P / Bovine

Mari Akiyama et al.
Preprint,  (2024)

publication_link

 

hr_line

Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis

IHC-P / Mouse

Jing Chang et al.
Front Pharmacol.,  (2023)

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Oral Administration of Rhamnan Sulfate from Monostroma nitidum Suppresses Atherosclerosis in ApoE-Deficient Mice Fed a High-Fat Diet

IHC-Fr / Mouse

Masahiro Terasawa et al.
Cells.,  (2023)

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Farrerol prevents Angiotensin II-induced cardiac remodeling in vivo and in vitro

WB / Mouse

Jian He et al.
Front Pharmacol.,  (2023)

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Elastic Fibers and F-Box and WD-40 Domain-Containing Protein 2 in Bovine Periosteum and Blood Vessels

IHC-P / Cattle

Mari Akiyama et al.
Biomimetics (Basel).,  (2023)

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CD11b mediates hypertensive cardiac remodeling by regulating macrophage infiltration and polarization

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"Two-birds-one-stone" colon-targeted nanomedicine treats ulcerative colitis via remodeling immune microenvironment and anti-fibrosis

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Jiaxin Zhang et al.
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ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1α-Mediated Mitochondrial Homeostasis

ICC/IF / Human

Pengfei Zhu et al.
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Inhibition of Angiogenesis and Tumor Progression of Mk-0429, an Integrin Αvβ3 Antagonist, on Oral Squamous Cell Carcinoma

IHC-P / Human

Takayuki Nakagawa et al.
Research Square,  (2022)

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Inhibition of angiogenesis and tumor progression of MK-0429, an integrin αvβ3 antagonist, on oral squamous cell carcinoma

IHC-P / Human

Takayuki Nakagawa et al.
J Cancer Res Clin Oncol.,  (2022)

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Blocking VCAM-1 ameliorates hypertensive cardiac remodeling by impeding macrophage infiltration

IHC-P / Mouse

Ze-Yang Qiu  et al.
Front Pharmacol,  (2022)

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VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.

IHC-P / Mouse

Qiu-Yue Lin et al.
Clin Transl Med.,  (2021)

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Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α

WB / Rat

Chen Ying-Ying et al.
Preprint.,  (2019)

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