anti-alpha Tubulin acetyl (Lys40) antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes alpha Tubulin acetyl (Lys40)
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4A8
  • 靶点名称: alpha Tubulin
  • 抗原物种: Human
  • 抗原: Synthetic peptide around acetylated Lys40 of Human alpha Tubulin.
  • Brand:
CAT.NO. : ARG66217
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes alpha Tubulin acetyl (Lys40)
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号4A8
靶点名称alpha Tubulin
抗原物种Human
抗原Synthetic peptide around acetylated Lys40 of Human alpha Tubulin.
偶联标记Un-conjugated
別名TUBA1; ALS22; Tubulin alpha-4A chain; Testis-specific alpha-tubulin; Alpha-tubulin 1; Tubulin alpha-1 chain; Tubulin H2-alpha; H2-ALPHA
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:100
WB1:1000 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol and 0.5% BSA
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 22145 Mouse TUBA4A

GeneID: 316531 Rat TUBA4A

GeneID: 7277 Human TUBA4A

基因名称TUBA4A
全名tubulin, alpha 4a
背景介绍Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
生物功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
预测分子量50 kDa
翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.

Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability. [UniProt]
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