anti-ApoJ / Clusterin antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes ApoJ / Clusterin
  • 反应物种: Hu
  • 预测物种: Dog
  • 应用: ICC/IF, IHC-P, WB
  • 特异性: This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ApoJ / Clusterin
  • Brand:
CAT.NO. : ARG63834
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes ApoJ / Clusterin
反应物种Hu
预测物种Dog
应用ICC/IF, IHC-P, WB
特异性This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称ApoJ / Clusterin
抗原物种Human
抗原C-EKALQEYRKKHREE
偶联标记Un-conjugated
別名Clusterin; Apolipoprotein J; SGP-2; SP-40; APOJ; ApoJbeta; CLU2; CLU1; NA1/NA2; TRPM2; CLI; SGP2; Apo-J; TRPM-2; ApoJalpha; Aging-associated gene 4 protein; APO-J; Complement cytolysis inhibitor a chain; Ku70-binding protein 1; Complement cytolysis inhibitor b chain; Testosterone-repressed prostate message 2; Complement-associated protein SP-40,40; KUB1; AAG4; Complement cytolysis inhibitor
应用说明
应用建议
应用推荐稀释比
ICC/IF10 µg/ml
IHC-P2.5 µg/ml
WB0.03 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Human colon
实际分子量~ 37 kDa
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 1191 Human CLU

Swiss-port # P10909 Human Clusterin

背景介绍The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
研究领域Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量52 kDa
翻译后修饰Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
检测图片 (3)
  • ARG63834 anti-ApoJ / Clusterin antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63834 anti-ApoJ / Clusterin antibody (green) at 10 µg/ml for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG63834 anti-ApoJ / Clusterin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63834 anti-ApoJ / Clusterin antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG63834 anti-ApoJ / Clusterin antibody WB image

    Western blot: 35 µg of Human colon lysate (in RIPA buffer) stained with ARG63834 anti-ApoJ / Clusterin antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

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