anti-ATP1B1 / Na+ K+ ATPase beta 1 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes ATP1B1 / Na+ K+ ATPase beta 1
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Cow, Dog, Pig
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ATP1B1 / Na+ K+ ATPase beta 1
  • Brand:
CAT.NO. : ARG65270
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes ATP1B1 / Na+ K+ ATPase beta 1
反应物种Hu, Ms, Rat
预测物种Cow, Dog, Pig
应用IHC-P, WB
特异性This antibody is expected to recognize both reported isoforms (NP_001668.1; NP_001001787.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称ATP1B1 / Na+ K+ ATPase beta 1
抗原物种Human
抗原C-KTEISFRPNDPKSYE
偶联标记Un-conjugated
別名Sodium/potassium-transporting ATPase subunit beta-1; Sodium/potassium-dependent ATPase subunit beta-1; ATP1B; Na+ K+ ATPase beta 1; Na K ATPase beta 1; sodium potassium ATPase beta 1; ATPase Na+ K+ beta 1; ATPase Na K beta 1; ATPase sodium potassium beta 1
应用说明
应用建议
应用推荐稀释比
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11931 Mouse ATP1B1

GeneID: 25650 Rat ATP1B1

GeneID: 481 Human ATP1B1

背景介绍The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010]
研究领域Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量35 kDa
检测图片 (2)
  • ARG65270 anti-ATP1B1 / Na+ K+ ATPase beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65270 anti-ATP1B1 / Na+ K+ ATPase beta 1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG65270 anti-ATP1B1 / Na+ K+ ATPase beta 1 antibody WB image

    Western blot: 35 µg of Rat retina lysate (in RIPA buffer) stained with ARG65270 anti-ATP1B1 / Na+ K+ ATPase beta 1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.

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