anti-ATP5J antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG58280
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes ATP5J |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Mk |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | ATP5J |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 28-56 (Center) of Human ATP5J. |
| 偶联标记 | Un-conjugated |
| 別名 | F6; ATP5; ATPM; ATPase subunit F6; CF6; ATP synthase-coupling factor 6, mitochondrial; ATP5A |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Mouse stomach |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ATP5J |
| 全名 | ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 |
| 背景介绍 | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.[provided by RefSeq, Jun 2010] |
| 生物功能 | Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. [UniProt] |
| 细胞定位 | Mitochondrion. Mitochondrion inner membrane. [UniProt] |
| 预测分子量 | 13 kDa |
检测图片 (3)
ARG58280 anti-ATP5J antibody ICC/IF image
Immunofluorescence: U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min). Cells were stained with ARG58280 anti-ATP5J antibody (green) at 1:25 dilution, 1 hour at 37°C. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). DAPI (blue) for nuclear staining.
ARG58280 anti-ATP5J antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG58280 anti-ATP5J antibody.
ARG58280 anti-ATP5J antibody WB image
Western blot: 35 µg of Mouse stomach lysate stained with ARG58280 anti-ATP5J antibody.
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