anti-BCKDK antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
-
Brand:
CAT.NO. : ARG43079
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Size:
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes BCKDK |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BCKDK |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 21-70 of Human BCKDK. |
| 偶联标记 | Un-conjugated |
| 別名 | BCKD-kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; BDK; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKDHKIN; BCKDKD; EC 2.7.11.4 |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.2), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | BCKDK |
| 全名 | branched chain ketoacid dehydrogenase kinase |
| 背景介绍 | The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] |
| 生物功能 | Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex. [UniProt] |
| 细胞定位 | Mitochondrion matrix. Mitochondrion. [UniProt] |
| 预测分子量 | 46 kDa |
| 翻译后修饰 | Autophosphorylated. [UniProt] |
检测图片 (3)
ARG43079 anti-BCKDK antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil carcinoma tissue stained with ARG43079 anti-BCKDK antibody at 1:50 dilution.
ARG43079 anti-BCKDK antibody WB image
Western blot: 40 µg of C6, AML-12, HEK293T and L-02 whole cell lysates stained with ARG43079 anti-BCKDK antibody at 1:500 dilution.
ARG43079 anti-BCKDK antibody IP image
Immunoprecipitation: HEK293T cell lysate using ARG43079 anti-BCKDK antibody (lane 2 and 3) and Nonspecific IgG Control (lane 4 and 5). Lane 1 is 30% input. Western blot was stained with ARG43079 anti-BCKDK antibody.
↑: Supernatant
↓: Deposition
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