anti-Beclin 1 antibody [45E8]

CAT.NO. : ARG57142

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Size:

50 μl

Product Details

概述

产品描述	Mouse Monoclonal antibody [45E8] recognizes Beclin 1
反应物种	Hu
应用	FACS, ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	45E8
同位型	IgG2b, kappa
靶点名称	Beclin 1
抗原物种	Human
抗原	Recombinant fragment around aa. 1-450 of Human Beclin-1
偶联标记	Un-conjugated
別名	BECN1; Beclin 1; VPS30; ATG6; Coiled-Coil Myosin-Like BCL2-Interacting Protein; Beclin 1, Autophagy Related; Beclin-1; Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein); Beclin 1 (Coiled-Coil, Moesin-Like BCL2-Interacting Protein); ATG6 Autophagy Related 6 Homolog (S. Cerevisiae); Testis Secretory Sperm-Binding Protein Li 215e; ATG6 Autophagy Related 6 Homolog; Beclin1-Gamma; Protein GT197; Beclin1-Beta; Beclin1; GT197

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 8678 Human BECN1 Swiss-port # Q14457 Human Beclin-1
基因名称	BECN1
全名	beclin 1, autophagy related
背景介绍	This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能	Protects against infection by a neurovirulent strain of Sindbis virus. May play a role in antiviral host defense. [UniProt]
细胞定位	Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus. [UniProt]
产品亮点	Related products: Beclin 1 antibodies; Beclin 1 Duos / Panels;
研究领域	Cancer antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Autophagy Study antibody
预测分子量	52 kDa
翻译后修饰	Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation. [UniProt]

检测图片 (4)

ARG57142 anti-Beclin 1 antibody [45E8] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57142 anti-Beclin 1 antibody [45E8] FACS image
Flow Cytometry: HeLa cell line stained with ARG57142 anti-Beclin 1 antibody [45E8] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57142 anti-Beclin 1 antibody [45E8] WB image
Western blot: 10 ng of 1) Recombinant Human BECN1 protein, and 2) Balb/3T3 cell lysates stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:5000.
ARG57142 anti-Beclin 1 antibody [45E8] WB image
Western blot: 10 µg of 1) 293T, and 2) BECN1 Transfected 293T cell lysate stained with ARG57142 anti-Beclin 1 antibody [45E8] at 1:1000.

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