anti-beta Catenin antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG52651
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes beta Catenin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | beta Catenin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide from C-terminus (768-781) of the beta catenin protein. |
| 偶联标记 | Un-conjugated |
| 別名 | CTNNB; armadillo; MRD19; Catenin beta-1; Beta-catenin |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | Breast Carcinoma, A431 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Immunogen affinity purified |
| 缓冲液 | PBS (pH 7.6), 1% BSA and |
| 抗菌剂 | |
| 稳定剂 | 1% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 背景介绍 | The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated with E-cadherin at cellular junctions. β-catenin also binds to N-cadherin and co-immunoprecipitates with APC. Cadherin/catenin complexes are linked to the cytoskeleton via a direct association between α-actinin and α-catenin. Increases tyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion. |
| 细胞定位 | Cytoplasm, Membrane |
| 产品亮点 | Related Antibody Duos and Panels: ARG30144 Phospho beta Catenin Antibody Panel (Total, pS33, pS37, pT41/pS45) Related products: beta Catenin antibodies; beta Catenin Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Besides tumor suppression, what’s p53 busy for during embryogenesis? Wnt / beta-catenin signaling for gastric fundus specification |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation (By similarity). S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. Deacetylated at Lys-49 by SIRT1. |
检测图片 (2)
ARG52651 anti-beta Catenin antibody WB image
Western blot: 30 µg of 1) 293T, 2) 3T3, and 3) Mouse liver lysate stained with ARG52651 anti-beta Catenin antibody at 1:500 dilution.
ARG52651 anti-Catenin-beta antibody IHC-P image
Immunohistochemistry: Human Breast Carcinoma stained with Catenin-beta antibody (ARG52651)
参考文献
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WB / Mouse
Narasin inhibits tumor metastasis and growth of ERα‑positive breast cancer cells by inactivation of the TGF‑β/SMAD3 and IL‑6/STAT3 signaling pathways
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Ubiquitin specific peptidase 5 enhances STAT3 signaling and promotes migration and invasion in Pancreatic Cancer.
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