anti-beta Tubulin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes beta Tubulin
  • 反应物种: Hu, Ms, Rat, Mk
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: beta Tubulin
  • 抗原物种: Human
  • 抗原: Synthetic peptide derived from Human beta Tubulin.
  • Brand:
CAT.NO. : ARG58689
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes beta Tubulin
反应物种Hu, Ms, Rat, Mk
应用ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称beta Tubulin
抗原物种Human
抗原Synthetic peptide derived from Human beta Tubulin.
偶联标记Un-conjugated
別名OK/SW-cl.56; CDCBM6; Tubulin beta chain; M40; TUBB5; Tubulin beta-5 chain; TUBB1
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:3000 - 1:10000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量50 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 203068 Human TUBB

GeneID: 574113 Monkey TUBB

Swiss-port # P07437 Human Tubulin beta chain

Swiss-port # P69895 Monkey Tubulin beta chain

基因名称TUBB
全名tubulin, beta class I
背景介绍This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13. [provided by RefSeq, Jun 2014]
生物功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [UniProt]
细胞定位Cytoplasm > Cytoskeleton. [UniProt]
研究领域Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody
预测分子量50 kDa
翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. [UniProt]
检测图片 (6)
  • ARG58689 anti-beta Tubulin antibody ICC/IF image

    Immunofluorescence: NIH/3T3 cells stained with ARG58689 anti-beta Tubulin antibody.

  • ARG58689 anti-beta Tubulin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human glioma stained with ARG58689 anti-beta Tubulin antibody.

  • ARG58689 anti-beta Tubulin antibody WB image

    Western blot: MCF-7, COS-1, Jurkat and HeLa cell lysates stained with ARG58689 anti-beta Tubulin antibody.

  • ARG58689 anti-beta Tubulin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG58689 anti-beta Tubulin antibody.

  • ARG58689 anti-beta Tubulin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse kidney stained with ARG58689 anti-beta Tubulin antibody.

  • ARG58689 anti-beta Tubulin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus cancer stained with ARG58689 anti-beta Tubulin antibody.

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