anti-BLNK / SLP65 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes BLNK / SLP65 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_037446.1; NP_001107566.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | BLNK / SLP65 |
| 抗原物种 | Human |
| 抗原 | C-KDSTRLKYAVKVS |
| 偶联标记 | Un-conjugated |
| 別名 | SLP65; BLNK-S; B-cell linker protein; bca; B-cell adapter containing a Src homology 2 domain protein; SLP-65; AGM4; LY57; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; BASH; B-cell adapter containing a SH2 domain protein |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 50 kDa |
| 翻译后修饰 | Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways. |
ARG63184 anti-BLNK / SLP65 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63184 anti-BLNK / SLP65 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG63184 anti-BLNK / SLP65 antibody WB image
Western blot: 1). Mock transfection; 2) BLNK (RC202488) expressing plasmid transfected HEK293 cell lysate standed with ARG63184 anti-BLNK / SLP65 antibody.
ARG63184 anti-BLNK / SLP65 antibody WB image
Western blot: 35 µg of Daudi cell lysate (in RIPA buffer) stained with ARG63184 anti-BLNK / SLP65 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
ARG63184 anti-BLNK / SLP65 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Daudi cells permeabilized with 0.5% Triton. Cells were stained with ARG63184 anti-BLNK / SLP65 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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