Anti-BRAF V600E Rabbit Monoclonal Antibody [Clone RM8]; BRAF(V600E); 31-1042-00; 31-1042-00-S; 31-1042-02
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Key features and details
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Product Details
| Product Name | BRAF V600E Rabbit Monoclonal Antibody, Clone RM8 |
| Product Description | Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E |
| Catalog No. | 31-1042-00; 31-1042-00-S; 31-1042-02 |
| Clone Name | RM8 |
| Product Data Sheet | BRAF V600E Rabbit Monoclonal Antibody Data Sheet Biotinylated BRAF V600E Rabbit Monoclonal Antibody Data Sheet |
| Specificity | RM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF. |
| Immunogen | A peptide corresponding to BRAF V600E mutant |
| Application | Immunohistochemistry Immunocytochemistry Western Blot ELISA Flow Cytometry |
| Protocols | BRAF V600E IHC Protocol |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg; 50 µg; Biotinylated 50 µg |
| Concentration | 1 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Immunohistochemistry (IHC): 0.5 µg/mL – 5 µg/mL; Immunocytochemistry (ICC): 0.5 µg/mL – 5 µg/mL; Western Blot (WB): 0.5 µg/mL – 2 µg/mL; ELISA: 0.5 µg/mL – 2 µg/mL; |
| References for BRAF V600E Mutant antibody [RM8] | Labib M et al. Tracking the expression of therapeutic protein targets in rare cells by antibody-mediated nanoparticle labelling and magnetic sorting. Nat Biomed Eng. (2020), 10.1038/s41551-020-0590-1. FC; Human. Read more (PubMed: 32719513) Crespo-Rodriguez E et al. Combining BRAF inhibition with oncolytic herpes simplex virus enhances the immune-mediated antitumor therapy of BRAF-mutant thyroid cancer. J Immunother Cancer. (2020), 10.1136/jitc-2020-000698. WB; Human. Read more (PubMed: 32759235) Martin AM et al. PD-L1 Expression in Pediatric Low-Grade Gliomas Is Independent of BRAF V600E Mutational Status. J Neuropathol Exp Neurol. (2020), 10.1093/jnen/nlz119. WB; Human. Read more (PubMed: 31819973) Shirsat PM et al. Low frequency of BRAF V600E immunoexpression in mandibular ameloblastomas: An institutional study. J Oral Maxillofac Pathol. (2018), 10.4103/jomfp.JOMFP_174_17. IHC; Human. Read more (PubMed: 30651680) Usman H et al. Result Analysis of BRAF V600E Gene Mutation Using Molecular and Immunohistochemistry Detection in Acral Malignant Melanoma. Asian Pacific Journal of Cancer Care. (2018), 10.31557/APJCC.2018.3.3.43. IHC; Human. Read more (WAOCP) Hansen C et al. Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection. Proc Natl Acad Sci USA. (2017), 10.1073/pnas.1708148114. ELISA, WB; Human. Read more (PubMed: 28893984) Krishnamurthy A et al. Clinical utility of immunohistochemistry using the novel anti-BRAF V600E antibody (clone RM8) for detection of the BRAF V600E mutant protein in papillary thyroid cancers. Int J Mol ImmunoOncol. (2018) 10.18203/issn.2456-3994.IntJMolImmunoOncol20180471. IHC-P; Human. Read more (IJMIO: ISSN: 2456-3994) Gouravan S et al. Preclinical Evaluation of Vemurafenib as Therapy for BRAFV600E Mutated Sarcomas. Int J Mol Sci. (2018) 10.3390/ijms19040969. WB; Human. Read more (PubMed: 29570692) Yang H et al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene (2017) 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969) ZM Shaban et al. BRAFV600E Mutation in Gliomas in Iraqi Patients Immunohistochemical Study. ijcocr (2017) 10.11648/j.ijcocr.20170206.11. IHC; Human. Read More |
 | Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 2 µg/mL. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 0.5 µg/mL. Allele specific PCR validated to be Negative for BRAF V600E. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded Thyroid Carcinoma tissue using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 0.5 µg/mL.Allele specific PCR validated to be Positive for BRAF V600E. |
 | Western Blot analysis of A375 cell (BRAF V600E) and HEK293 cell (WT) lysates using anti-BRAF V600E rabbit monoclonal antibody, Clone RM8, at 2 µg/mL. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human cell line sections. |
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