anti-CaMKII phospho (Thr286) antibody [22B1]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [22B1] recognizes CaMKII phospho (Thr286) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, IHC, IP, WB |
| 特异性 | Detects phosphorylated CaMKII from rat tissues. Monoclonal antibody 22B1 (anti-phosphoCaMKII is specific for α and β subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in β). |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 22B1 |
| 同位型 | IgG |
| 靶点名称 | CaMKII |
| 抗原物种 | Rat |
| 抗原 | Phosphospecific peptide around Thr286 of Rat CaMKII (NP_037052.1). |
| 偶联标记 | Un-conjugated |
| 別名 | CAMKA; CaMK-II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; EC 2.7.11.17 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | Rat brain tissue extract. | ||||||||||||
| 实际分子量 | ~50 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G affinity purified |
| 缓冲液 | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | Camk2a |
| 全名 | calcium/calmodulin-dependent protein kinase II alpha |
| 背景介绍 | The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| 生物功能 | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. [UniProt] |
| 产品亮点 | Related products: anti-CaMKII phospho (Thr286) antibody [22B1]; |
| 研究领域 | Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 54 kDa |
ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC-P image
Immunohistochemistry: Bouin's fixed paraffin-embedded backskin sections of transgenic mice- muscle, and hair follicle stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image
Western blot: Rat brain lysate stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] ICC/IF image
Immunofluorescence: Rat hippocampal neurons stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1].
ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] IHC image
Immunohistochemistry: Formalin-fixed Human colon carcinoma stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] at 1:5000 for 12 hours at 4°C. Secondary antibody: Biotin Goat anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 min at RT. Magnification: 40x.
ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1] WB image
Western blot: 1) Ventricles lysate from 14-Day-Old normal Mouse, 2) Ventricles lysate from 14-Day-Old CaM overexpressing transgenic Mouse line 2295, 3) Ventricles lysate from 14-Day-Old CaM-8 (mutant CaM) expressing transgenic Mouse line 4466, stained with ARG20520 anti-CaMKII phospho (Thr286) antibody [22B1]. (PMID: 10935538).
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WB / Rat
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