anti-CBF beta antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes CBF beta
  • 反应物种: Hu, Ms
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: CBF beta
  • 抗原物种: Human
  • 抗原: KLH-conjugated synthetic peptide corresponding to aa. 61-90 (Center) of Human CBF beta.
  • Brand:
CAT.NO. : ARG55069
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes CBF beta
反应物种Hu, Ms
应用FACS, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称CBF beta
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 61-90 (Center) of Human CBF beta.
偶联标记Un-conjugated
別名PEBP2-beta; Polyomavirus enhancer-binding protein 2 beta subunit; CBF-beta; SL3-3 enhancer factor 1 subunit beta; PEA2-beta; PEBP2B; SL3/AKV core-binding factor beta subunit; Core-binding factor subunit beta
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-PAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照K562
属性
形式Liquid
纯化Purification with Protein A and immunogen peptide.
缓冲液PBS and 0.09% (W/V) Sodium azide
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12400 Mouse CBFB

GeneID: 865 Human CBFB

Swiss-port # Q08024 Mouse Core-binding factor subunit beta

Swiss-port # Q13951 Human Core-binding factor subunit beta

基因名称CBFB
全名core-binding factor, beta subunit
背景介绍The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1. [UniProt]
细胞定位Nucleus.
研究领域Gene Regulation antibody
预测分子量22 kDa
检测图片 (3)
  • ARG55069 anti-CBF beta antibody ICC/IF image

    Immunofluorescence: 293 cells stained with ARG55069 anti-CBF beta antibody.

  • ARG55069 anti-CBF beta antibody WB image

    Western blot: 35 µg of K562 cell lysate stained with ARG55069 anti-CBF beta antibody.

  • ARG55069 anti-CBF beta antibody FACS image

    Flow Cytometry: HL-60 cells stained with ARG55069 anti-CBF beta antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.

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