anti-CD11b antibody [CA16.3E10]
![anti-CD11b antibody [CA16.3E10]](/upload/image/products/ARG23377_FACS_1_210_205.jpg)
![anti-CD11b antibody [CA16.3E10]](/upload/image/products/ARG23377_FACS_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG23377
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [CA16.3E10] recognizes CD11b Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family. CD11b forms one of the possible alpha chains of the canine leukocyte adhesion complexes (LeuCAMs), these contain a common 95 kDa β chain (CD18) non-covalently bound to either a 150 kDa (CD11c), 165 kDa (CD11b) or 180 kDa (CD11a) α chain (Moore et al. 1990. The CD11/CD18 complex is also known as the CR3 receptor.Canine CD11b is expressed by granulocytes, monocytes, NK cells and some macrophages. Mouse anti Dog CD11b (CA16.3E10) has been used tio evaluate the effect of anaesthetic administration of CD11b expression on canine neutrophils (Maeda et al. 2010) demonstrating attenuation of CD11b expression at high concentrations administered lidocaine hydrochloride and reduced adhesion of neutrophils to endothelium. |
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| 反应物种 | Bov, Cat, Dog, Goat, Pig |
| 应用 | FACS, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CA16.3E10 |
| 同位型 | IgG1 |
| 靶点名称 | CD11b |
| 抗原物种 | Dog |
| 抗原 | Affinity purified beta-2 integrins from splenic lysate |
| 偶联标记 | Un-conjugated |
| 別名 | MAC1A; CR3A; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Integrin alpha-M; MAC-1; CD11 antigen-like family member B; Leukocyte adhesion receptor MO1; MO1A; SLEB6; Neutrophil adherence receptor; CD antigen CD11b; CD11B |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Tissue Culture Supernatant. |
| 缓冲液 | PBS and 0.1% Sodium azide. |
| 抗菌剂 | 0.1% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ITGAM |
|---|---|
| 全名 | integrin, alpha M (complement component 3 receptor 3 subunit) |
| 背景介绍 | CD11b (integrin alpha M chain): Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | CD11b: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:9558116, PubMed:20008295). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils. May play a role in mast cell development. Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development. [UniProt] |
| 产品亮点 | Related products: CD11b antibodies; CD11b Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| 研究领域 | MDSC Marker antibody; Myeloid-derived suppressor cell antibody |
| 预测分子量 | 127 kDa |
检测图片 (1)
ARG23377 anti-CD11b antibody [CA16.3E10] FACS image
Flow Cytometry: Figure A. FITC conjugated Mouse anti Canine CD45 and purified Mouse IgG1 isotype control detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Mouse anti Canine CD45 and ARG23377 anti-CD11b antibody [CA16.3E10] detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed Canine blood gated on mononuclear cells.
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