anti-CD154 / CD40L antibody [24-31]

CAT.NO. : ARG41606

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Size:

100 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [24-31] recognizes CD154 / CD40L
反应物种	Hu, NHuPrm
应用	FACS, ICC
宿主	Mouse
克隆	Monoclonal
克隆号	24-31
同位型	IgG1
靶点名称	CD154 / CD40L
抗原物种	Human
抗原	Human CD154 fusion protein.
偶联标记	Un-conjugated
別名	TNFSF5; IMD3; T-cell antigen Gp39; HIGM1; CD40-L; gp39; CD40 ligand; Tumor necrosis factor ligand superfamily member 5; CD40L; CD154; TRAP; CD antigen CD154; hCD40L; IGM; T-BAM; TNF-related activation protein

应用说明

应用建议

应用	推荐稀释比
FACS	2 - 6 µg/ml
ICC	Assay-dependent

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS and 15 mM Sodium azide.
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 959 Human CD40LG Swiss-port # P29965 Human CD40 ligand
基因名称	CD40LG
全名	CD40 ligand
背景介绍	The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
生物功能	Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. [UniProt]
细胞定位	Cell membrane; Single-pass type II membrane protein. Cell surface. CD40 ligand, soluble form: Secreted. [UniProt]
预测分子量	29 kDa
翻译后修饰	The soluble form derives from the membrane form by proteolytic processing. N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. Not O-glycosylated. [UniProt]

检测图片 (2)

ARG41606 anti-CD154 / CD40L antibody [24-31] FACS image
Flow Cytometry: Human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained with ARG41606 anti-CD154 / CD40L antibody [24-31] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.
ARG41606 anti-CD154 / CD40L antibody [24-31] FACS image
Flow Cytometry: Separation of human CD154 positive cells (red-filled) from CD154 negative cells (black-dashed). Stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained with ARG41606 anti-CD154 / CD40L antibody [24-31] at 2 µg/ml dilution, followed by APC-conjugated Goat anti-Mouse antibody.

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