anti-CD163 antibody [ED2]

Key features and details

产品描述: Mouse Monoclonal antibody [ED2] recognizes CD163Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
反应物种: Rat
应用: FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
宿主: Mouse
克隆: Monoclonal
克隆号: ED2
同位型: IgG1
靶点名称: CD163
抗原物种: Rat

Brand:

CAT.NO. : ARG23390

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Size:

50 μg

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Product Details

概述

产品描述	Mouse Monoclonal antibody [ED2] recognizes CD163 Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007). Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
反应物种	Rat
应用	FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
宿主	Mouse
克隆	Monoclonal
克隆号	ED2
同位型	IgG1
靶点名称	CD163
抗原物种	Rat
抗原	Rat Spleen cell homogenate.
偶联标记	Un-conjugated
別名	sCD163; M130; Scavenger receptor cysteine-rich type 1 protein M130; MM130; CD antigen CD163; Hemoglobin scavenger receptor

应用说明

应用建议

应用	推荐稀释比
FACS	1:10 - 1:100
ICC/IF	Assay-dependent
IHC-Fr	1:50 - 1:100
IHC-P	Assay-dependent
IP	Assay-dependent
WB	Assay-dependent

应用说明

IHC-P: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS and 0.09% Sodium azide.
抗菌剂	0.09% Sodium azide
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称	CD163
全名	CD163 molecule
背景介绍	CD163 protein is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
生物功能	CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. [UniProt]
产品亮点	Related products: CD163 antibodies; CD163 ELISA Kits; CD163 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Anti-SerpinB9 therapy, a new strategy for cancer therapy RIP1 activation and pathogenesis of NASH
研究领域	M1/M2/TAM Marker antibody; Macrophage Marker antibody; M2 Macrophage Marker antibody
预测分子量	125 kDa
翻译后修饰	A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. [UniProt]

检测图片 (7)

ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Acetone fixed, cryostat sectioned Rat spleen stained with ARG23390 anti-CD163 antibody [ED2].
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Low power).
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Medium power).
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (Medium power).
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2] followed by HRP-conjugated Goat anti Mouse IgG1 as a detection reagent. (High power).
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained blue using DAPI. (Low power).
ARG23390 anti-CD163 antibody [ED2] IHC-Fr image
Immunohistochemistry: Rat lymph node cryosection stained with ARG23390 anti-CD163 antibody [ED2], red in A and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counter-stained blue using DAPI. (High power).

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