anti-CD177 antibody [MEM-166] (FITC)

Key features and details

  • 产品描述: FITC-conjugated Mouse Monoclonal antibody [MEM-166] recognizes CD177
  • 反应物种: Hu, NHuPrm
  • 应用: FACS
  • 特异性: The clone MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes. HLDA VI; WS Code M M17HLDA VI; WS Code BP 309
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: MEM-166
  • 同位型: IgG1
  • 靶点名称: CD177
  • Brand:
CAT.NO. : ARG62749
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Product Details
概述
产品描述FITC-conjugated Mouse Monoclonal antibody [MEM-166] recognizes CD177
反应物种Hu, NHuPrm
应用FACS
特异性The clone MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.
HLDA VI; WS Code M M17
HLDA VI; WS Code BP 309
宿主Mouse
克隆Monoclonal
克隆号MEM-166
同位型IgG1
靶点名称CD177
抗原物种Human
抗原Human granulocytes
偶联标记FITC
別名NB1 glycoprotein; PRV-1; PRV1; NB1; CD177 antigen; Human neutrophil alloantigen 2a; NB1 GP; HNA2A; Polycythemia rubra vera protein 1; CD antigen CD177; HNA-2a
应用说明
应用建议
应用推荐稀释比
FACS20 µl / 10^6 cells
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化说明The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂15 mM Sodium azide
稳定剂0.2% (w/v) high-grade protease free BSA
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 57126 Human CD177

Swiss-port # Q8N6Q3 Human CD177 antigen

基因名称CD177
全名CD177 molecule
背景介绍CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
研究领域Immune System antibody
预测分子量46 kDa
翻译后修饰N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding).
检测图片 (2)
  • ARG62749 anti-CD177 antibody [MEM-166] (FITC) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG62749 anti-CD177 antibody [MEM-166] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

  • ARG62749 anti-CD177 antibody [MEM-166] (FITC) FACS image

    Flow Cytometry: Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed). Human peripheral whole blood stained with ARG62749 anti-CD177 antibody [MEM-166] (FITC) (20 µl reagent / 100 µl of peripheral whole blood).

参考文献

克隆号文献

Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177.

WB, FACS / Human

Hu N et al.
PLoS One.,  (2014)

publication_link

 

hr_line

The Thr224Asn mutation in the VPS45 gene is associated with the congenital neutropenia and primary myelofibrosis of infancy.

IHC-P / Human

Stepensky P et al.
Blood.,  (2013)

publication_link

 

hr_line

Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells.

Drukker M et al.
Nat Biotechnol.,  (2012)

publication_link

 

hr_line

Decreased CXCR1 and CXCR2 expression on neutrophils in anti-neutrophil cytoplasmic autoantibody-associated vasculitides potentially increases neutrophil adhesion and impairs migration.

FACS / Human

Hu N et al.
Arthritis Res Ther.,  (2011)

publication_link

 

hr_line

CXCR3-dependent accumulation and activation of perivascular macrophages is necessary for homeostatic arterial remodeling to hemodynamic stresses.

FACS / Human

Zhou J et al.
J Exp Med.,  (2010)

publication_link

 

hr_line
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