anti-CD18 / LFA1 beta antibody [MEM-48]
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Key features and details
- 产品描述:
- 反应物种:
- 不反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
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Brand:
CAT.NO. : ARG62751
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-48] recognizes CD18 / LFA1 beta |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Dog |
| 应用 | FACS, FuncSt, IHC-Fr, IHC-P, IP, WB |
| 特异性 | The clone MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-48 |
| 同位型 | IgG1 |
| 靶点名称 | CD18 / LFA1 beta |
| 抗原 | Leukocytes of a patient suffering from a LGL-type leukemia. |
| 偶联标记 | Un-conjugated |
| 別名 | MF17; LAD; CD antigen CD18; MFI7; MAC-1; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; LCAMB; Integrin beta-2; Complement receptor C3 subunit beta; LFA-1; CD18 |
应用说明
| 应用建议 |
| ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Sample preparation: buffer with laurylmaltoside, 2x non-reducing SDS. Application note: Non-reducing condition. SDS-PAGE (6% separating gel). Functional studies: The clone MEM-48 induces high-affinity conformation of LFA-1 complex. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| 阳性对照 | WB: Kg-1a human leukemia cells IHC-P: Spleen and microglia |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ITGB2 |
| 全名 | integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) |
| 背景介绍 | CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. |
| 生物功能 | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. [UniProt] |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters (PubMed:11700305). Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins (PubMed:11700305, PubMed:16301335). |
检测图片 (2)
ARG62751 anti-CD18 / LFA1 beta antibody [MEM-48] WB image
Western blot: 1) KG-1a cell lysate, and 2) HPB-ALL Human peripheral blood T cell leukemia cell lysate stained with ARG62751 anti-CD18 / LFA1 beta antibody [MEM-48].
ARG62751 anti-CD18 / LFA1 beta antibody [MEM-48] IHC-P image
Immunohistochemistry: Human spleen (paraffin sections) stained with ARG62751 anti-CD18 / LFA1 beta antibody [MEM-48].
参考文献
克隆号文献
Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
ICC/IF /
Analysis on the relevance of asthma susceptibility with the alteration of integrin β 4 expression.
WB / Human
Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow.
NKp80 defines and stimulates a reactive subset of CD8 T cells.
Human leukocytes contain a large pool of free forms of CD18.
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