anti-CD180 / RP105 antibody [G28-8] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [G28-8] recognizes CD180
反应物种: Hu
应用: FACS
特异性: The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
宿主: Mouse
克隆: Monoclonal
克隆号: G28-8
同位型: IgG1
靶点名称: CD180 / RP105

CAT.NO. : ARG54247

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Size:

50 tests

Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [G28-8] recognizes CD180
反应物种	Hu
应用	FACS
特异性	The mouse monoclonal antibody G288 recognizes CD180, a 95105 kDa TLRlike glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
宿主	Mouse
克隆	Monoclonal
克隆号	G28-8
同位型	IgG1
靶点名称	CD180 / RP105
抗原物种	Human
抗原	Human tonsillar B cells
偶联标记	PE
別名	LY64; Radioprotective 105 kDa protein; CD180 antigen; CD antigen CD180; Lymphocyte antigen 64; RP105; Ly78

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4064 Human CD180 Swiss-port # Q99467 Human CD180 antigen
基因名称	CD180
全名	CD180 molecule
背景介绍	CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
生物功能	May cooperate with MD-1 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) in B-cells. Leads to NF-kappa-B activation. Also involved in the life/death decision of B-cells (By similarity). [UniProt]
研究领域	Immune System antibody; Microbiology and Infectious Disease antibody
预测分子量	74 kDa

检测图片 (1)

ARG54247 anti-CD180 / RP105 antibody [G28-8] (PE) FACS image
Flow Cytometry: Human peripheral blood cells stained with ARG54247 anti-CD180 / RP105 antibody [G28-8] (PE).

参考文献

克隆号文献

Caspase activity is required for stimulated B lymphocytes to enter the cell cycle.

Olson NE et al.
J Immunol., (2003)