anti-CD204 / MSR1 antibody [2F8] (low endotoxin)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG22423
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Product Details
概述
| 产品描述 | Azide free and low endotoxin Rat Monoclonal antibody [2F8] recognizes CD204 / MSR1 This antibody recognizes the murine scavenger receptor class A (SRA), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993). Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000). |
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| 反应物种 | Ms, Cfsh, Pig |
| 应用 | ELISA, FACS, FuncSt, IHC-Fr, IP, WB |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | 2F8 |
| 同位型 | IgG2b |
| 靶点名称 | CD204 / MSR1 |
| 抗原物种 | Mouse |
| 抗原 | Raw 264 cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | Macrophage scavenger receptor types I and II; SR-A; SCARA1; Macrophage acetylated LDL receptor I and II; SRA; CD antigen CD204; Scavenger receptor class A member 1; CD204; phSR1; phSR2 |
应用说明
| 应用建议 |
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| 应用说明 | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. WB: This product recognises CD204 in J774 cells under non-reduced conditions only. FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 纯化说明 | Low endotoxin |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P30204 Mouse Macrophage scavenger receptor types I and II |
|---|---|
| 基因名称 | Msr1 |
| 全名 | macrophage scavenger receptor 1 |
| 背景介绍 | This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008] |
| 生物功能 | Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize acetylated LDL. [UniProt] |
| 预测分子量 | 50 kDa |
检测图片 (2)
ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin) FACS image
Flow Cytometry: Mouse peritoneal macrophages stained with ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin).
ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin) WB image
Western blot: J774 cell lysate (non reduced) stained with ARG22423 anti-CD204 / MSR1 antibody [2F8] (low endotoxin).
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