anti-CD22 antibody [MEM-01] (PE)

Key features and details

  • 产品描述: PE-conjugated Mouse Monoclonal antibody [MEM-01] recognizes CD22
  • 反应物种: Hu, NHuPrm
  • 应用: FACS
  • 特异性: The clone MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: MEM-01
  • 同位型: IgG1
  • 靶点名称: CD22
  • Brand:
CAT.NO. : ARG53793
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Product Details
概述
产品描述PE-conjugated Mouse Monoclonal antibody [MEM-01] recognizes CD22
反应物种Hu, NHuPrm
应用FACS
特异性The clone MEM-01 reacts with CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.
MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
宿主Mouse
克隆Monoclonal
克隆号MEM-01
同位型IgG1
靶点名称CD22
抗原物种Human
抗原Raji Burkitt's lymphoma cell line
偶联标记PE
別名B-lymphocyte cell adhesion molecule; B-cell receptor CD22; T-cell surface antigen Leu-14; BL-CAM; SIGLEC-2; Sialic acid-binding Ig-like lectin 2; Siglec-2; CD antigen CD22; SIGLEC2
应用说明
应用建议
应用推荐稀释比
FACS20 µl / 10^6 cells
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化说明The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂15 mM Sodium azide
稳定剂0.2% (w/v) high-grade protease free BSA
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 933 Human CD22

Swiss-port # P20273 Human B-cell receptor CD22

基因名称CD22
全名CD22 molecule
背景介绍CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
生物功能Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. [UniProt]
研究领域Cancer antibody; Developmental Biology antibody; Immune System antibody; Immature B Cell Marker antibody
预测分子量95 kDa
翻译后修饰Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN.
检测图片 (2)
  • ARG53793 anti-CD22 antibody [MEM-01] (PE) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG53793 anti-CD22 antibody [MEM-01] (PE) (20 µl reagent / 100 µl of peripheral whole blood).

  • ARG53793 anti-CD22 antibody [MEM-01] (PE) FACS image

    Flow Cytometry: Separation of human CD22 positive lymphocytes (red-filled) from CD22 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG53793 anti-CD22 antibody [MEM-01] (PE) (20 µl reagent / 100 µl of peripheral whole blood).

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