anti-CD25 antibody [7D4] (PE-Cyanine 7)

Key features and details

  • 产品描述: PE-Cyanine 7-conjugated Rat Monoclonal antibody [7D4] recognizes CD25
  • 反应物种: Ms
  • 应用: BL, Depletion, FACS, IHC-Fr, Neut
  • 特异性: Mouse CD25. The clone 7D4 reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by The clone 3C7. While The clone 7D4 does not block the binding of IL-2 to CD25, when used in combination with The clone 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
  • 宿主: Rat
  • 克隆: Monoclonal
  • 克隆号: 7D4
  • 同位型: IgM, kappa
  • 靶点名称: CD25
  • Brand:
CAT.NO. : ARG20886
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Product Details
概述
产品描述PE-Cyanine 7-conjugated Rat Monoclonal antibody [7D4] recognizes CD25
反应物种Ms
应用BL, Depletion, FACS, IHC-Fr, Neut
特异性Mouse CD25. The clone 7D4 reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by The clone 3C7. While The clone 7D4 does not block the binding of IL-2 to CD25, when used in combination with The clone 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
宿主Rat
克隆Monoclonal
克隆号7D4
同位型IgM, kappa
靶点名称CD25
抗原物种Mouse
抗原IL-2 dependent BALB/c helper T cell clone HT2
偶联标记PE-Cyanine 7
別名IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10
应用说明
应用建议
应用推荐稀释比
BLAssay-dependent
DepletionAssay-dependent
FACS
IHC-FrAssay-dependent
NeutAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
缓冲液PBS, 0.1% Sodium azide and Sucrose.
抗菌剂0.1% Sodium azide
稳定剂Sucrose
浓度0.1 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 16184 Mouse IL2RA

Swiss-port # P01590 Mouse Interleukin-2 receptor subunit alpha

基因名称IL2RA
全名interleukin 2 receptor, alpha chain
背景介绍The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
生物功能Receptor for interleukin-2. [UniProt]
研究领域Immune System antibody; Pre-B Cell Marker antibody
预测分子量31 kDa
检测图片 (2)
  • ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7) FACS image

    Flow Cytometry: Con-A-stimulated BALB/c Mouse splenocytes stained with ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7).

  • ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7) FACS image

    Flow Cytometry: C57BL/6 Mouse cytotoxic T lymphocyte cell line CTLL-2 was stained with ARG20886 anti-CD25 antibody [7D4] (PE-Cyanine 7).

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