anti-CD25 antibody [MEM-140] (Biotin)

产品描述: Biotin-conjugated Mouse Monoclonal antibody [MEM-140] recognizes CD25
反应物种: Hu
应用: FACS
特异性: The clone MEM-140 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.HLDA VI; WS Code C C-54
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-140
同位型: IgM
靶点名称: CD25

CAT.NO. : ARG62783

US＄ Please choose

Size:

100 μg

Product Details

概述

产品描述	Biotin-conjugated Mouse Monoclonal antibody [MEM-140] recognizes CD25
反应物种	Hu
应用	FACS
特异性	The clone MEM-140 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4⁺ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes. HLDA VI; WS Code C C-54
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-140
同位型	IgM
靶点名称	CD25
抗原	PHA-activated peripheral blood leucocytes
偶联标记	Biotin
別名	IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

应用说明

应用建议

应用	推荐稀释比
FACS	1 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
缓冲液	TBS (pH 8.0) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3559 Human IL2RA Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha
基因名称	IL2RA
全名	interleukin 2 receptor, alpha
背景介绍	CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
生物功能	Receptor for interleukin-2. [UniProt]
研究领域	Immune System antibody; Pre-B Cell Marker antibody
预测分子量	31 kDa

检测图片 (1)

ARG62783 anti-CD25 antibody [MEM-140] (Biotin) FACS image
Flow Cytometry: PHA-activated Human PBMC stained with ARG62783 anti-CD25 antibody [MEM-140] (Biotin), followed by Streptavidin (APC).

New Products