anti-CD279 / PD-1 antibody [4D6]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [4D6] recognizes CD279 / PD-1
  • 反应物种: Hu
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4D6
  • 同位型: IgG1
  • 靶点名称: CD279 / PD-1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56131
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Product Details
概述
产品描述Mouse Monoclonal antibody [4D6] recognizes CD279 / PD-1
反应物种Hu
应用ICC/IF, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号4D6
同位型IgG1
靶点名称CD279 / PD-1
抗原物种Human
抗原Extracellular domain of Human PD-1.
偶联标记Un-conjugated
別名hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1
应用说明
应用建议
应用推荐稀释比
ICC/IF5 µg/ml
IHC-P20 µg/ml
WB0.25 - 0.5 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS and 0.02% Sodium azide
抗菌剂0.02% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 5133 Human PDCD1

Swiss-port # Q15116 Human Programmed cell death protein 1

基因名称PDCD1
全名programmed cell death 1
背景介绍CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
生物功能CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
细胞定位Membrane
产品亮点Related products:
PD-1 antibodies; PD-1 ELISA Kits; PD-1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
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预测分子量32 kDa
检测图片 (3)
  • ARG56131 anti-CD279 / PD-1 antibody [4D6] ICC/IF image

    Immunocytochemistry: Transfected 293 cells stained with ARG56131 anti-CD279 / PD-1 antibody [4D6] at 5 µg/ml dilution. Lower left: Same cells stained with control Mouse IgG antibody at 5 µg/ml dilution.

  • ARG56131 anti-CD279 / PD-1 antibody [4D6] IHC-P image

    Immunohistochemistry: (A) Human tonsil tissue, (B) Human lymph node tissue, and (C) Human spleen tissue stained with ARG56131 anti-CD279 / PD-1 antibody [4D6] at 5 µg/ml dilution. (D) Human tonsil tissue stained with control Mouse IgG antibody at 5 µg/ml dilution.

  • ARG56131 anti-CD279 / PD-1 antibody [4D6] ICC/IF image

    Immunocytochemistry: Transfected 293 cells stained with ARG56131 anti-CD279 / PD-1 antibody [4D6] at 5 µg/ml dilution. Lower left: Same cells stained with control Mouse IgG antibody at 5 µg/ml dilution.

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