anti-CD284 / TLR4 antibody [HTA125] (PE)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG22987
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [HTA125] recognizes CD284 / TLR4 |
|---|---|
| 反应物种 | Hu, Dog, Gpig, Pig, R. Mk |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | HTA125 |
| 同位型 | IgG2a |
| 靶点名称 | CD284 / TLR4 |
| 抗原物种 | Human |
| 抗原 | Ba/F3 cell line expressing TLR4 (CD284). |
| 偶联标记 | PE |
| 別名 | CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TLR4 |
| 全名 | toll-like receptor 4 |
| 背景介绍 | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [UniProt] |
| 产品亮点 | Related products: TLR4 antibodies; TLR4 ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Detecting exosomal HMGB1 for ICD research |
| 预测分子量 | 96 kDa |
| 翻译后修饰 | N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. |
检测图片 (1)
ARG22987 anti-CD284 / TLR4 antibody [HTA125] (PE) FACS image
Flow Cytometry: U937 cells stained with ARG22987 anti-CD284 / TLR4 antibody [HTA125] (PE).
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