anti-CD32 antibody [3D3] (PE)

产品描述: PE-conjugated Mouse Monoclonal antibody [3D3] recognizes CD32
反应物种: Hu
应用: FACS
特异性: The mouse monoclonal antibody 3D3 recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
宿主: Mouse
克隆: Monoclonal
克隆号: 3D3
同位型: IgG1
靶点名称: CD32

CAT.NO. : ARG54201

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50 tests

Product Details

概述

产品描述	PE-conjugated Mouse Monoclonal antibody [3D3] recognizes CD32
反应物种	Hu
应用	FACS
特异性	The mouse monoclonal antibody 3D3 recognizes CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
宿主	Mouse
克隆	Monoclonal
克隆号	3D3
同位型	IgG1
靶点名称	CD32
抗原物种	Human
抗原	purified glycosylated recombinant human FcgammaRIIa2
偶联标记	PE
別名	Fc-gamma RII-a; CD antigen CD32; FcGR; IgG Fc receptor II-a; FCGR2A1; CD32A; FCGR2; Low affinity immunoglobulin gamma Fc region receptor II-a; FcRII-a; Fc-gamma-RIIa; FCG2; IGFR2; CD32; CDw32

应用说明

应用建议

应用	推荐稀释比
FACS	10 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2212 Human FCGR2A Swiss-port # P12318 Human Low affinity immunoglobulin gamma Fc region receptor II-a
基因名称	FCGR2A
全名	Fc fragment of IgG, low affinity IIa, receptor (CD32)
背景介绍	CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).
生物功能	Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. [UniProt]
研究领域	Developmental Biology antibody; Immune System antibody
预测分子量	35 kDa
翻译后修饰	Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

检测图片 (2)

ARG54201 anti-CD32 antibody [3D3] (PE) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG54201 anti-CD32 antibody [3D3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).
ARG54201 anti-CD32 antibody [3D3] (PE) FACS image
Flow Cytometry: Separation of human CD32 positive lymphocytes (red-filled) from CD32 negative lymphocytes (black-dashed). Human peripheral whole blood stained with ARG54201 anti-CD32 antibody [3D3] (PE) (10 µl reagent / 100 µl of peripheral whole blood).

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