anti-CD32 antibody [AT10]
![anti-CD32 antibody [AT10]](/upload/image/products/ARG22888_FACS_1_210_205.jpg)
![anti-CD32 antibody [AT10]](/upload/image/products/ARG22888_FACS_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG22888
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [AT10] recognizes CD32 Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997). |
|---|---|
| 反应物种 | Hu, Dog, Pig, R. Mk |
| 应用 | FACS, IHC-Fr, IP |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | AT10 |
| 同位型 | IgG1 |
| 靶点名称 | CD32 |
| 抗原物种 | Human |
| 抗原 | K562 cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | Fc-gamma RII-a; CD antigen CD32; FcGR; IgG Fc receptor II-a; FCGR2A1; CD32A; FCGR2; Low affinity immunoglobulin gamma Fc region receptor II-a; FcRII-a; Fc-gamma-RIIa; FCG2; IGFR2; CD32; CDw32 |
应用说明
| 应用建议 |
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| 应用说明 | Functional study: This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or cells or 100 µl whole blood. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P12318 Human Low affinity immunoglobulin gamma Fc region receptor II-a |
|---|---|
| 基因名称 | FCGR2A |
| 全名 | Fc fragment of IgG, low affinity IIa, receptor (CD32) |
| 背景介绍 | This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
| 生物功能 | Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. [UniProt] |
| 预测分子量 | 35 kDa |
| 翻译后修饰 | Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. |
检测图片 (1)
ARG22888 anti-CD32 antibody [AT10] FACS image
Flow Cytometry: Human peripheral blood granulocytes stained with ARG22888 anti-CD32 antibody [AT10].
参考文献
The antibodies against the A137R protein drive antibody-dependent enhancement of African swine fever virus infection in porcine alveolar macrophages
Blocking / Pig
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