anti-CD34 antibody [4H11 (APG)] (Biotin)

产品描述: Biotin-conjugated Mouse Monoclonal antibody [4H11 (APG)] recognizes CD34
反应物种: Hu
应用: FACS
特异性: The clone 4H11(APG) reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. 4H11(APG) completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line.HLDA VI; WS Code M MA58
宿主: Mouse
克隆: Monoclonal
克隆号: 4H11 (APG)
同位型: IgG1
靶点名称: CD34

CAT.NO. : ARG62819

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Size:

100 μg

Product Details

概述

产品描述	Biotin-conjugated Mouse Monoclonal antibody [4H11 (APG)] recognizes CD34
反应物种	Hu
应用	FACS
特异性	The clone 4H11(APG) reacts with Class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. 4H11(APG) completely blocks binding of Class II antibody QBEnd10 and Class III antibodies BIRMA K3 and 8G12 on KG1a cell line. HLDA VI; WS Code M MA58
宿主	Mouse
克隆	Monoclonal
克隆号	4H11 (APG)
同位型	IgG1
靶点名称	CD34
抗原物种	Human
抗原	Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
偶联标记	Biotin
別名	Hematopoietic progenitor cell antigen CD34; CD antigen CD34

应用说明

应用建议

应用	推荐稀释比
FACS	1 - 2 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 947 Human CD34 Swiss-port # P28906 Human Hematopoietic progenitor cell antigen CD34
基因名称	CD34
全名	CD34 molecule
背景介绍	CD34 protein may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
生物功能	CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. [UniProt]
研究领域	Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Pro-B Cell Marker antibody; Endothelial Cell Marker antibody; Angiogenesis Study antibody
预测分子量	41 kDa
翻译后修饰	Highly glycosylated. Phosphorylated on serine residues by PKC.

检测图片 (1)

ARG62819 anti-CD34 antibody [4H11 (APG)] (Biotin) FACS image
Flow Cytometry: Human peripheral blood stained with ARG62819 anti-CD34 antibody [4H11 (APG)] (Biotin), followed by Streptavidin (APC). CD34+ cells (red); CD34- cells (black-dashed).

参考文献

克隆号文献

Label-free impedance detection of low levels of circulating endothelial progenitor cells for point-of-care diagnosis.

ELISA / Human

Ng SY et al.
Biosens Bioelectron., (2010)