anti-CD43 antibody [MEM-59]
![anti-CD43 antibody [MEM-59]](/upload/image/products/MEM-59_2_210_205.jpg)
![anti-CD43 antibody [MEM-59]](/upload/image/products/MEM-59_1.jpg)
![anti-CD43 antibody [MEM-59]](/upload/image/products/MEM-59_2.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
CAT.NO. : ARG62848
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [MEM-59] recognizes CD43 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, FuncSt, IHC-P, IP, WB |
| 特异性 | The clone MEM-59 recognizes neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes. HLDA IV; WS Code NL 604 HLDA V; WS Code AS S290 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-59 |
| 同位型 | IgG1 |
| 靶点名称 | CD43 |
| 抗原物种 | Human |
| 抗原 | Human T lymphocytes. |
| 偶联标记 | Un-conjugated |
| 別名 | CD antigen CD43; B-cell differentiation antigen LP-3; Sialophorin; Cd43; Galgp; A630014B01Rik; Ly-48; Lymphocyte antigen 48; Ly48; Leukocyte sialoglycoprotein; Leukosialin |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | Functional studies: The clone MEM-59 activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||
| 阳性对照 | IHC-P: Spleen, thymus and tonsil. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPN |
| 全名 | sialophorin |
| 背景介绍 | CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions. |
| 生物功能 | One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity). [UniProt] |
| 产品亮点 | Related products: CD43 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Lymphoma |
| 研究领域 | Developmental Biology antibody; Immune System antibody |
| 预测分子量 | 40 kDa |
检测图片 (2)
ARG62848 anti-CD43 antibody [MEM-59] WB image
Western blot: 1) Human, 2) Canine, and 3) Porcine PBL lysates stained with ARG62848 anti-CD43 antibody [MEM-59].
ARG62848 anti-CD43 antibody [MEM-59] IHC-P image
Immunohistochemistry: Human spleen (paraffin sections) stained with ARG62848 anti-CD43 antibody [MEM-59].
参考文献
克隆号文献
CD24 expression identifies teratogen-sensitive fetal neural stem cell subpopulations: evidence from developmental ethanol exposure and orthotopic cell transfer models.
FACS / Mouse
The carboxy terminus of NBS1 is required for induction of apoptosis by the MRE11 complex.
WB / Mouse
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.
MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation.
CD59 molecule: a second ligand for CD2 in T cell adhesion.
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