anti-CD44 antibody [MEM-263] (FITC)

产品描述: FITC-conjugated Mouse Monoclonal antibody [MEM-263] recognizes CD44
反应物种: Hu, Dog, Pig
应用: FACS
特异性: The clone MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.HLDA III; WS Code T 155
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-263
同位型: IgG1
靶点名称: CD44

CAT.NO. : ARG62854

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Size:

100 tests

Product Details

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [MEM-263] recognizes CD44
反应物种	Hu, Dog, Pig
应用	FACS
特异性	The clone MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. HLDA III; WS Code T 155
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-263
同位型	IgG1
靶点名称	CD44
抗原	COS-7 cells (African Green Monkey).
偶联标记	FITC
別名	MDU2; MDU3; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; Extracellular matrix receptor III; PGP-I; Epican; CDW44; Phagocytic glycoprotein 1; Pgp1; HUTCH-I; MC56; Hyaluronate receptor; CD antigen CD44; Heparan sulfate proteoglycan; CD44 antigen; LHR; IN; HCELL; Phagocytic glycoprotein I; PGP-1; CSPG8; MIC4; ECMR-III; CDw44

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 960 Human CD44 Swiss-port # P16070 Human CD44 antigen
背景介绍	CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.
研究领域	Cancer antibody; Developmental Biology antibody; Immune System antibody; Chondrogenesis Study antibody
预测分子量	82 kDa
翻译后修饰	Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. N- and O-glycosylated. O-glycosylation contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). It is uncertain if O-glycosylation occurs on Thr-637 or Thr-638. Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

检测图片 (1)

ARG62854 anti-CD44 antibody [MEM-263] (FITC) FACS image
Flow Cytometry: Human peripheral blood stained with ARG62854 anti-CD44 antibody [MEM-263] (FITC).

参考文献

克隆号文献

Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs.

Linard C et al.
Stem Cells Transl Med., (2013)