anti-CD45 antibody [MEM-28] (PE)
![anti-CD45 antibody [MEM-28] (PE)](/upload/image/products/ARG53855_FACS_2_201117_210_205.jpg)
![anti-CD45 antibody [MEM-28] (PE)](/upload/image/products/ARG53855_FACS_1_201117.jpg)
![anti-CD45 antibody [MEM-28] (PE)](/upload/image/products/ARG53855_FACS_2_201117.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 不反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
-
Brand:
Product Details
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [MEM-28] recognizes CD45 |
|---|---|
| 反应物种 | Hu |
| 不反应物种 | Hrs |
| 应用 | FACS |
| 特异性 | The clone MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III; WS Code NL 833a |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-28 |
| 同位型 | IgG1 |
| 靶点名称 | CD45 |
| 抗原物种 | Human |
| 抗原 | Human thymocytes and T lymphocytes. |
| 偶联标记 | PE |
| 別名 | LY5; GP180; Receptor-type tyrosine-protein phosphatase C; CD45; L-CA; CD antigen CD45; Leukocyte common antigen; CD45R; LCA; T200; EC 3.1.3.48; B220 |
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P08575 Human Receptor-type tyrosine-protein phosphatase C |
|---|---|
| 基因名称 | PTPRC |
| 全名 | protein tyrosine phosphatase, receptor type, C |
| 背景介绍 | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012] |
| 生物功能 | CD45: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. [UniProt] |
| 产品亮点 | Related products: CD45 antibodies; CD45 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| 研究领域 | Developmental Biology antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody; Mouse Inflammatory Cell Marker antibody;B Cell Marker antibody |
| 预测分子量 | 147 kDa |
| 翻译后修饰 | Heavily N- and O-glycosylated. |
ARG53855 anti-CD45 antibody [MEM-28] (PE) FACS image
Flow Cytometry: Human peripheral whole blood stained with ARG53855 anti-CD45 antibody [MEM-28] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
ARG53855 anti-CD45 antibody [MEM-28] (PE) FACS image
Flow Cytometry: Separation of human lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed). Human peripheral whole blood stained with ARG53855 anti-CD45 antibody [MEM-28] (PE) (20 µl reagent / 100 µl of peripheral whole blood).
克隆号文献
Adult human glia, pericytes and meningeal fibroblasts respond similarly to IFNy but not to TGFβ1 or M-CSF.
ICC/IF / Human
Individual profiling of circulating tumor cell composition and therapeutic outcome in patients with hepatocellular carcinoma.
ICC/IF / Human
M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia.
ICC/IF /
Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
FACS / Human
HIV Type 1 Nef is released from infected cells in CD45(+) microvesicles and is present in the plasma of HIV-infected individuals.
WB / Human
IL-6 signaling in psoriasis prevents immune suppression by regulatory T cells.
IHC-P / Human
A novel fluorescence-based method in forensic science for the detection of blood in situ.
ICC/IF / Human
The cytoplasmic tail of CD45 is released from activated phagocytes and can act as an inhibitory messenger for T cells.
WB, FACS / Human
New Products
