anti-CD50 / ICAM3 antibody [ICAM3/1019]
![anti-CD50 / ICAM3 antibody [ICAM3/1019]](/upload/image/products/ARG43671_WB_210_205.jpg)
![anti-CD50 / ICAM3 antibody [ICAM3/1019]](/upload/image/products/ARG43671_IHCP.jpg)
![anti-CD50 / ICAM3 antibody [ICAM3/1019]](/upload/image/products/ARG43671_WB.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
CAT.NO. : ARG43671
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [ICAM3/1019] recognizes CD50 / ICAM3 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | ICAM3/1019 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | CD50 / ICAM3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to CD50 / ICAM3 antibody. |
| 偶联标记 | Un-conjugated |
| Protein Full name | Intercellular adhesion molecule 3 |
| 別名 | CD50; CD antigen CD50; Intercellular adhesion molecule 3; ICAM-3; ICAM-R; CDw50; CDW50 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | Antigen retrieval for IHC-P: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | 100-160 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ICAM3 |
| 全名 | intercellular adhesion molecule 3 |
| 背景介绍 | The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq, Jul 2008] |
| 生物功能 | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2. [UniProt] |
| 细胞定位 | Cell surface |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
检测图片 (2)
ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human Tonsil tissue stained with ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] at 1:100 dilution. Antigen Retrieval: Heat mediated was performed using 10 mM citrate buffer pH 6.0
ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] WB image
Western blot: HL-60 cell lysate ARG43671 anti-CD50 / ICAM3 antibody [ICAM3/1019] at 1:100 dilution
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