anti-CD71 / Transferrin Receptor antibody [MEM-75] (APC)
![anti-CD71 / Transferrin Receptor antibody [MEM-75] (APC)](/upload/image/products/ARG53895_FACS_1_210_205.jpg)
![anti-CD71 / Transferrin Receptor antibody [MEM-75] (APC)](/upload/image/products/ARG53895_FACS_1.jpg)
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CAT.NO. : ARG53895
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Product Details
概述
| 产品描述 | APC-conjugated Mouse Monoclonal antibody [MEM-75] recognizes CD71 / Transferrin Receptor |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS |
| 特异性 | The clone MEM-75 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. MEM-75 does not block binding of transferrin to the receptor. HLDA IV; WS Code A 45 HLDA V; WS Code T T-165 |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MEM-75 |
| 同位型 | IgG1 |
| 靶点名称 | CD71 / Transferrin Receptor |
| 抗原物种 | Human |
| 抗原 | NALM-6 human pre-B cell line |
| 偶联标记 | APC |
| 別名 | TFR1; CD antigen CD71; CD71; T9; p90; TR; Trfr; Transferrin receptor protein 1; TRFR; sTfR; TfR1; TfR; TFR |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TFRC |
| 全名 | transferrin receptor |
| 背景介绍 | CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. |
| 生物功能 | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. |
检测图片 (1)
ARG53895 anti-CD71 / Transferrin Receptor antibody [MEM-75] (APC) FACS image
Flow Cytometry: K562 cells (red) and Human lymphocytes (grey, negative control) stained with ARG53895 anti-CD71 / Transferrin Receptor antibody [MEM-75] (APC).
参考文献
克隆号文献
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