anti-CD88 / C5AR1 antibody [S5/1] (PE)
![anti-CD88 / C5AR1 antibody [S5/1] (PE)](/upload/image/products/ARG54294_FACS_1_201117_210_205.jpg)
![anti-CD88 / C5AR1 antibody [S5/1] (PE)](/upload/image/products/ARG54294_FACS_1_201117.jpg)
Key features and details
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- 应用:
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- 靶点名称:
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CAT.NO. : ARG54294
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Product Details
概述
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [S5/1] recognizes CD88 / C5AR1 |
|---|---|
| 反应物种 | Hu, Bov, Frt, Rb |
| 应用 | FACS |
| 特异性 | The mouse monoclonal antibody S5/1 recognizes the CD88 protein, a 43 kDa receptor of C5a component of the complement cascade. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | S5/1 |
| 同位型 | IgG2a |
| 靶点名称 | CD88 / C5AR1 |
| 抗原物种 | Human |
| 抗原 | Recombinant N-terminal peptide (Asp15-Asp27) of human C5aR |
| 偶联标记 | PE |
| 別名 | CD88; C5R1; C5AR; CD antigen CD88; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5A; C5aR; C5a-R |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化说明 | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| 缓冲液 | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| 抗菌剂 | 15 mM Sodium azide |
| 稳定剂 | 0.2% (w/v) high-grade protease free BSA |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P21730 Human C5a anaphylatoxin chemotactic receptor 1 |
|---|---|
| 基因名称 | C5AR1 |
| 全名 | complement component 5a receptor 1 |
| 背景介绍 | CD88 / C5aR is a G protein-coupledseven membrane-spanning protein serving as a receptor for C5a component of the complement cascade, and is expressed mainly by monocytes, macrophages, neutrophils, eosinophils, and mast cells, but also e.g. by hepatocytes, glial cells, vascular endothelial cells, or cardiomyocytes. The binding of C5a to CD88 is associated with inflammatory response, including superoxide anion production, chemotaxis, and increased production of acute phase proteins. Expression of CD88 on synovial mast cells and their C5a-mediated degranulation plays a role in pathogenesis of rheumatoid arthritis. |
| 生物功能 | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a (PubMed:1847994, PubMed:8182049, PubMed:7622471, PubMed:9553099, PubMed:10636859, PubMed:15153520). The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events (PubMed:8182049, PubMed:7622471, PubMed:9553099). Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production (PubMed:10636859, PubMed:15153520). [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sulfation plays a critical role in the association of C5aR with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small a small peptide agonist (PubMed:11342590). Sulfation at Tyr-14 is important for CHIPS binding (PubMed:21706042). Phosphorylated on serine residues in response to C5a binding, resulting in internalization of the receptor and short-term desensitization to the ligand. The key residues involved in this process are Ser-334 and Ser-338. |
检测图片 (1)
ARG54294 anti-CD88 / C5AR1 antibody [S5/1] (PE) FACS image
Flow Cytometry: Human peripheral blood stained with ARG54294 anti-CD88 / C5AR1 antibody [S5/1] (PE).
参考文献
克隆号文献
C5a and its receptors in human anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis.
IHC-P / Human
C5a mediates peripheral blood neutrophil dysfunction in critically ill patients.
Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
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