anti-CD8b antibody [PPT23] (PE)
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | PE-conjugated Mouse Monoclonal antibody [PPT23] recognizes CD8b Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells. Characterization of clone PPT23 has shown that in thymic tissue both CD8lo and CD8hi cells are recognized (Yang H & Parkhouse R.M. 1997). Inhibition studies have demonstrated that clone PPT23 recognizes a different epitope of on the CD8 beta chain to clone PPT22. |
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| 反应物种 | Pig |
| 应用 | FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PPT23 |
| 同位型 | IgG1 |
| 靶点名称 | CD8b |
| 抗原物种 | Pig |
| 抗原 | Porcine thymus membrane lysate. |
| 偶联标记 | PE |
| 別名 | LY3; CD8B1; CD antigen CD8b; LEU2; T-cell surface glycoprotein CD8 beta chain; P37; LYT3 |
| 应用建议 |
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| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 1% BSA and 5% Sucrose |
| 存放说明 | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 基因名称 | CD8B |
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| 全名 | CD8b molecule |
| 背景介绍 | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010] |
| 生物功能 | Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. [UniProt] |
| 产品亮点 | Related products: CD8 antibodies; CD8 ELISA Kits; CD8 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| 预测分子量 | 24 kDa |
| 翻译后修饰 | Phosphorylated as a consequence of T-cell activation. [UniProt] |
ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Porcine peripheral blood lymphocytes stained with ARG23177 anti-CD8b antibody [PPT23] (PE).
ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Figure A. FITC-conjugated Mouse anti Porcine CD5 and PE-conjugated Mouse IgG1 isotype control. Figure B. FITC-conjugated Mouse anti Porcine CD5 and ARG23177 anti-CD8b antibody [PPT23] (PE). All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.
ARG23177 anti-CD8b antibody [PPT23] (PE) FACS image
Flow Cytometry: Figure A. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse IgG2a isotype control. Figure B. ARG23177 anti-CD8b antibody [PPT23] (PE) and FITC-conjugated Mouse anti Porcine CD5. All experiments performed on red cell lysed Porcine blood gated on mononuclear cells.
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