anti-CD95 / Fas antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
-
Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes CD95 / Fas |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-Fr, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CD95 / Fas |
| 抗原物种 | Rat |
| 抗原 | Synthetic peptide around the N-terminus of Rat CD95. (YTDRKHYSDKCRRCAFCDEGHGL) |
| 偶联标记 | Un-conjugated |
| 別名 | CD95; Apoptosis-mediating surface antigen FAS; FAS1; Tumor necrosis factor receptor superfamily member 6; ALPS1A; APT1; FASTM; CD antigen CD95; APO-1; TNFRSF6; FASLG receptor; Apo-1 antigen |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.025% Sodium azide and 2.5% BSA. |
| 抗菌剂 | 0.025% Sodium azide |
| 稳定剂 | 2.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P25446 Mouse Tumor necrosis factor receptor superfamily member 6 Swiss-port # Q63199 Rat Tumor necrosis factor receptor superfamily member 6 |
|---|---|
| 基因名称 | Fas |
| 全名 | Fas cell surface death receptor |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011] |
| 生物功能 | Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein |
| 预测分子量 | 35 - 38 kDa (unmodified); 40 - 55 kDa (glycosylated) |
| 翻译后修饰 | N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. |
ARG56208 anti-CD95 / Fas antibody IHC-P image
Immunohistochemistry: Rat spleen tissue stained with ARG56208 anti-CD95 / Fas antibody.
Antigen retrieval: Boil the paraffin sections in 10mM citrate buffer, pH6, for 20 mins.
ARG56208 anti-CD95 / Fas antibody WB image
Western blot: Testing of Rat 1) liver, 2) spleen, 3) brain and 4) heart lysates stained with ARG56208 anti-CD95 / Fas antibody at 1 µg/ml dilution.
ARG56208 anti-CD95 / Fas antibody WB image
Western blot: Testing of Mouse 1) liver, 2) spleen, 3) brain, 4) kidney, 5) thymus, 6) lung, 7) Hepa 1-6, and 8) NIH 3T3 cell lysates stained with ARG56208 anti-CD95 / Fas antibody.
ARG56208 anti-CD95 / Fas antibody WB image
Western blot: 1) 10 ng, 2) 5 ng, and 3) 2.5 ng of recombinant Mouse protein stained with ARG56208 anti-CD95 / Fas antibody.
New Products
