anti-CDC25B phospho (Ser323) antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes CDC25B phospho (Ser323) |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | CDC25B |
| 抗原物种 | Human |
| 抗原 | Phosphospecific peptide around Ser323 of Human CDC25B. |
| 偶联标记 | Un-conjugated |
| 別名 | M-phase inducer phosphatase 2; EC 3.1.3.48; Dual specificity phosphatase Cdc25B |
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: High-pressure and temperature Tris/EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 实际分子量 | ~ 70 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CDC25B |
| 全名 | cell division cycle 25B |
| 背景介绍 | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008] |
| 生物功能 | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. [UniProt] |
| 预测分子量 | 65 kDa |
| 翻译后修饰 | Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. [UniProt] |
ARG66656 anti-CDC25B phospho (Ser323) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast cancer tissue stained with ARG66656 anti-CDC25B phospho (Ser323) antibody at 1:100 dilution, overnight at 4°C. Antigen Retrieval: High-pressure and temperature Tris/EDTA buffer (pH 8.0). Negative control (right): Antibody was pre-absorbed by phospho peptide.
ARG66656 anti-CDC25B phospho (Ser323) antibody WB image
Western blot: VEC cell lysate stained with ARG66656 anti-CDC25B phospho (Ser323) antibody at 1:1000 dilution.
ARG66656 anti-CDC25B phospho (Ser323) antibody WB image
Western blot: VEC, 293 and A549 cell lysates stained with ARG66656 anti-CDC25B phospho (Ser323) antibody at 1:1000 dilution.
ARG66656 anti-CDC25B phospho (Ser323) antibody WB image
Western blot: NIH/3T3 cells treated with PMA (125 ng/ml for 30 min). Cell lysates stained with ARG66656 anti-CDC25B phospho (Ser323) antibody. The lane on the right is blocked with the phospho peptide.
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