anti-Cyclin E1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes Cyclin E1 |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Cyclin E1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide within aa. 60-140 of Human Cyclin E1. |
| 偶联标记 | Un-conjugated |
| 別名 | pCCNE1; CCNE; G1/S-specific cyclin-E1 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | ~ 47 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | CCNE1 |
| 全名 | cyclin E1 |
| 背景介绍 | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available. [provided by RefSeq, Jul 2008] |
| 生物功能 | Essential for the control of the cell cycle at the G1/S (start) transition. [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. [UniProt] |
ARG66529 anti-Cyclin E1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG66529 anti-Cyclin E1 antibody. The picture on the right is blocked with the synthetic peptide.
ARG66529 anti-Cyclin E1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis stained with ARG66529 anti-Cyclin E1 antibody at 1:200 (4°C, overnight). Antigen Retrieval: High-pressure and temperature EDTA buffer (pH 8.0).
ARG66529 anti-Cyclin E1 antibody WB image
Western blot: MCF7 cell lysate stained with ARG66529 anti-Cyclin E1 antibody at 1:500 dilution.
ARG66529 anti-Cyclin E1 antibody IHC image
Immunohistochemistry: Rat spleen tissue stained with ARG66529 anti-Cyclin E1 antibody (red) at 1:200 (4°C, overnight). DAPI (blue) for nuclear staining.
ARG66529 anti-Cyclin E1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung carcinoma stained with ARG66529 anti-Cyclin E1 antibody. The picture on the right is blocked with the synthetic peptide.
ARG66529 anti-Cyclin E1 antibody WB image
Western blot: K562 cell lysate stained with ARG66529 anti-Cyclin E1 antibody. The lane on the right is blocked with the synthetic peptide.
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