anti-Cyclophilin B antibody [k2E2]

CAT.NO. : ARG56953

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Size:

50 μl

Product Details

概述

产品描述	Mouse Monoclonal antibody [k2E2] recognizes Cyclophilin B
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	k2E2
同位型	IgG1, kappa
靶点名称	Cyclophilin B
抗原物种	Human
抗原	Recombinant fragment around aa. 26-216 of Human Cyclophilin B.
偶联标记	Un-conjugated
別名	HEL-S-39; CYPB; PPIase B; CYP-S1; Cyclophilin B; SCYLP; EC 5.2.1.8; S-cyclophilin; OI9; Rotamase B; Peptidyl-prolyl cis-trans isomerase B

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5479 Human PPIB Swiss-port # P23284 Human Peptidyl-prolyl cis-trans isomerase B
基因名称	PPIB
全名	peptidylprolyl isomerase B (cyclophilin B)
背景介绍	The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009]
生物功能	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. [UniProt]
预测分子量	24 kDa

检测图片 (4)

ARG56953 anti-Cyclophilin B antibody [k2E2] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56953 anti-Cyclophilin B antibody [k2E2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG56953 anti-Cyclophilin B antibody [k2E2] WB image
Western blot: 30 µg HepG2 and HeLa cell lysates stained with ARG56953 anti-Cyclophilin B antibody [k2E2] at 1:1000.
ARG56953 anti-Cyclophilin B antibody [k2E2] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) K562 cell lysate, 3) 293T cell lysate, 4) A549 cell lysate, 5) MCF7 cell lysate, 6) SK-OV-3 cell lysate, 7) LnCap cell lysate stained with ARG56953 anti-Cyclophilin B antibody [k2E2] at 1:1000.
ARG56953 anti-Cyclophilin B antibody [k2E2] WB image
Western blot: 50 ng of 1) Cyclophilin B Recombinant Protein, 2) Cyclophilin A Recombinant Protein, 3) Cyclophilin D Recombinant Protein, 4) Cyclophilin E Recombinant Protein, 5) Cyclophilin F Recombinant Protein, 6) Cyclophilin G Recombinant Protein, 7) Cyclophilin H Recombinant Protein stained with ARG56953 anti-Cyclophilin B antibody [k2E2] at 1:1000.

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