anti-Cystatin B / Stefin B antibody [2F1]

CAT.NO. : ARG56951

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Size:

50 μl

Product Details

概述

产品描述	Mouse Monoclonal antibody [2F1] recognizes Cystatin B / Stefin B
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	2F1
同位型	IgG2b, kappa
靶点名称	Cystatin B / Stefin B
抗原物种	Human
抗原	Recombinant fragment around aa. 1-98 of Human Cystatin B / Stefin B.
偶联标记	Un-conjugated
別名	Liver thiol proteinase inhibitor; EPM1; CPI-B; EPM1A; Cystatin-B; Stefin-B; PME; CST6; ULD; STFB

应用说明

应用建议

应用	推荐稀释比
ICC/IF	Assay-dependent
WB	1:1000 - 1:2000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 1476 Human CSTB Swiss-port # P04080 Human Cystatin-B
基因名称	CSTB
全名	cystatin B (stefin B)
背景介绍	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). [provided by RefSeq, Jul 2008]
生物功能	This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. [UniProt]
预测分子量	11 kDa

检测图片 (4)

ARG56951 anti-Cystatin B / Stefin B antibody [2F1] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG56951 anti-Cystatin B / Stefin B antibody [2F1] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56951 anti-Cystatin B / Stefin B antibody [2F1] WB image
Western blot: 35 µg of A549 stained with ARG56951 anti-Cystatin B / Stefin B antibody [2F1] at 1:1000.
ARG56951 anti-Cystatin B / Stefin B antibody [2F1] ICC/IF image
Immunoflorescense: A549 cell line stained with ARG56951 anti-Cystatin B / Stefin B antibody [2F1] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56951 anti-Cystatin B / Stefin B antibody [2F1] WB image
Western blot: 40 µg of U-87MG cell lysate stained with ARG56951 anti-Cystatin B / Stefin B antibody [2F1] at 1:1000.

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